Modulation of metabolic activity of phagocytes by antihistamines
Identifieur interne : 000B51 ( Main/Exploration ); précédent : 000B50; suivant : 000B52Modulation of metabolic activity of phagocytes by antihistamines
Auteurs : Antonin Lojek [République tchèque] ; Milan [République tchèque] ; Michaela Pekarová [République tchèque] ; Gabriela Ambrožová [République tchèque] ; Ond Ej Vaší Ek [République tchèque] ; Jana Moravcová [République tchèque] ; Lukáš Kubala [République tchèque] ; Katarína Drábiková ; Viera Jan Inová ; Tomáš Pere Ko ; Jana Pe Ivová ; Tatiana Ma I Ková ; Radomír NosáSource :
- Interdisciplinary Toxicology [ 1337-6853 ] ; 2011-03-01.
English descriptors
Abstract
The purpose of the study was to investigate the effects of H1-antihistamines of the 1st generation (antazoline, bromadryl, brompheniramine, dithiaden, cyclizine, chlorcyclizine, chlorpheniramine, clemastine) and the 2nd generation (acrivastine, ketotifen, and loratadine) on the respiratory burst of phagocytes. Reactive oxygen species generation in neutrophils isolated from rat blood was measured using luminol-enhanced chemiluminescence. Changes in nitrite formation and iNOS protein expression by RAW 264.7 macrophages were analysed using Griess reaction and Western blotting. The antioxidative properties of drugs in cell-free systems were detected spectrophotometrically, luminometrically, fluorimetrically, and amperometrically. The majority of the H1-antihistamines tested (bromadryl, brompheniramine, chlorcyclizine, chlorpheniramine, clemastine, dithiaden, and ketotifen) exhibited a significant inhibitory effect on the chemiluminescence activity of phagocytes. H1-antihistamines did not show significant scavenging properties against superoxide anion and hydroxyl radical, thus this could not contribute to the inhibition of chemiluminescence. H1-antihistamines had a different ability to modulate nitric oxide production by LPS-stimulated macrophages. Bromadryl, clemastine, and dithiaden were the most effective since they inhibited iNOS expression, which was followed by a significant reduction in nitrite levels. H1-antihistamines had no scavenging activity against nitric oxide. It can be concluded that the effects observed in the H1-antihistamines tested are not mediated exclusively via H1-receptor pathway or by direct antioxidative properties. Based on our results, antihistamines not interfering with the microbicidal mechanisms of leukocytes (antazoline, acrivastine and cyclizine) could be used preferentially in infections. Other antihistamines should be used, under pathological conditions accompanied by the overproduction of reactive oxygen species.
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DOI: 10.2478/v10102-011-0004-z
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Reactive oxygen species generation in neutrophils isolated from rat blood was measured using luminol-enhanced chemiluminescence. Changes in nitrite formation and iNOS protein expression by RAW 264.7 macrophages were analysed using Griess reaction and Western blotting. The antioxidative properties of drugs in cell-free systems were detected spectrophotometrically, luminometrically, fluorimetrically, and amperometrically. The majority of the H1-antihistamines tested (bromadryl, brompheniramine, chlorcyclizine, chlorpheniramine, clemastine, dithiaden, and ketotifen) exhibited a significant inhibitory effect on the chemiluminescence activity of phagocytes. H1-antihistamines did not show significant scavenging properties against superoxide anion and hydroxyl radical, thus this could not contribute to the inhibition of chemiluminescence. H1-antihistamines had a different ability to modulate nitric oxide production by LPS-stimulated macrophages. Bromadryl, clemastine, and dithiaden were the most effective since they inhibited iNOS expression, which was followed by a significant reduction in nitrite levels. H1-antihistamines had no scavenging activity against nitric oxide. It can be concluded that the effects observed in the H1-antihistamines tested are not mediated exclusively via H1-receptor pathway or by direct antioxidative properties. Based on our results, antihistamines not interfering with the microbicidal mechanisms of leukocytes (antazoline, acrivastine and cyclizine) could be used preferentially in infections. Other antihistamines should be used, under pathological conditions accompanied by the overproduction of reactive oxygen species.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Drabikova, Katarina" sort="Drabikova, Katarina" uniqKey="Drabikova K" first="Katarína" last="Drábiková">Katarína Drábiková</name><name sortKey="Jan Inova, Viera" sort="Jan Inova, Viera" uniqKey="Jan Inova V" first="Viera" last="Jan Inová">Viera Jan Inová</name><name sortKey="Ma I Kova, Tatiana" sort="Ma I Kova, Tatiana" uniqKey="Ma I Kova T" first="Tatiana" last="Ma I Ková">Tatiana Ma I Ková</name><name sortKey="Nosa, Radomir" sort="Nosa, Radomir" uniqKey="Nosa R" first="Radomír" last="Nosá">Radomír Nosá</name><name sortKey="Pe Ivova, Jana" sort="Pe Ivova, Jana" uniqKey="Pe Ivova J" first="Jana" last="Pe Ivová">Jana Pe Ivová</name><name sortKey="Pere Ko, Tomas" sort="Pere Ko, Tomas" uniqKey="Pere Ko T" first="Tomáš" last="Pere Ko">Tomáš Pere Ko</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Lojek, Antonin" sort="Lojek, Antonin" uniqKey="Lojek A" first="Antonin" last="Lojek">Antonin Lojek</name></noRegion><name sortKey=", Milan" sort=", Milan" uniqKey=" M" first="Milan" last="">Milan </name><name sortKey="Ambrozova, Gabriela" sort="Ambrozova, Gabriela" uniqKey="Ambrozova G" first="Gabriela" last="Ambrožová">Gabriela Ambrožová</name><name sortKey="Kubala, Lukas" sort="Kubala, Lukas" uniqKey="Kubala L" first="Lukáš" last="Kubala">Lukáš Kubala</name><name sortKey="Moravcova, Jana" sort="Moravcova, Jana" uniqKey="Moravcova J" first="Jana" last="Moravcová">Jana Moravcová</name><name sortKey="Pekarova, Michaela" sort="Pekarova, Michaela" uniqKey="Pekarova M" first="Michaela" last="Pekarová">Michaela Pekarová</name><name sortKey="Vasi Ek, Ond Ej" sort="Vasi Ek, Ond Ej" uniqKey="Vasi Ek O" first="Ond Ej" last="Vaší Ek">Ond Ej Vaší Ek</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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